Assessment of the transcriptome quality of “multiplet” clusters.

(A–C) Violin plots outlining the number of features, number of counts, and proportion of mitochondrial genes, for the astrocyte (A), ensheathing (B), and cortex (C) glia cluster multiplets. Cluster identity is colour-coded and the cluster number is indicated on the x-axes. (A) Astrocyte multiplets showed no overall trend in transcriptome quality. (B) Ensheathing glia showed a decrease in transcriptome quality in clusters #5 and #7, compared to #3. (C) Cortex glia cluster #13 appears to be of a lower quality than #11. (D) GO analysis indicating the enriched biological processes in Astrocyte_1 (cluster #0) and Astrocyte_2 (cluster #2). The data underlying this figure can be found in S7 Data and on GitHub at https://github.com/VilFernandesLab/2022_DrosophilaGlialAtlas. (TIF)